|
KMID : 0613820100200020208
|
|
Journal of Life Science
2010 Volume.20 No. 2 p.208 ~ p.212
|
|
|
Development of Molecular Markers for Alternative Oxidase Synthesis Genes in Brassica rapa L.
|
|
Jeong Ye-sol
Chung Sang-Min
|
|
|
Abstract
|
|
|
|
The low and high temperature stress might affect the yield and quality of many crop species. Alternative oxidase (AOX) gene is known as factors related to stress resistance in plants. In order to develop molecular markers related to stress resistance in Chinese cabbage, fifteen ESTs sharing sequence similarity to arabidopsis AOX genes were found using Brassica rapa EST database from NCBI. The polymorphic DNA sequences using the ESTs were then screened between Chinese cabbage, ‘Chiifu’ and ‘Kenshin’. We found four ESTs that have either insertion or deletion between the two cultivars. These polymorphic sites were then targeted for development of the four PCR based molecular markers. These molecular markers developed in this study could be useful for a test of their relationship with abiotic stress resistance in Chinese cabbage.
|
|
|
KEYWORD
|
|
|
Chinese cabbage, abiotic stress, alternative oxidase, molecular marker
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|